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Decellularized extracellular matrix mediates tissue construction and regeneration
《医学前沿(英文)》 2022年 第16卷 第1期 页码 56-82 doi: 10.1007/s11684-021-0900-3
关键词: decellularized extracellular matrix 3D culture organoids tissue repair
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue
null
《医学前沿(英文)》 2013年 第7卷 第4期 页码 486-491 doi: 10.1007/s11684-013-0287-x
The purpose of this study was to compare cell growth characteristics, ciliated cell differentiation, and function of human nasal epithelial cells established as explant outgrowth cultures or dissociated tissue cultures. Human nasal mucosa of the uncinate process was obtained by endoscopy and epithelial cell cultures were established by explant outgrowth or dissociated tissue culture methods. Epithelial cell growth characteristics were observed by inverted phase contrast microscopy. Ciliated cell differentiation was detected by β-tubulin IV and ZO-1 immunocytochemistry. Basal and ATP-stimulated ciliary beat frequency (CBF) was measured using a high-speed digital microscopic imaging system. Both the explant and dissociated tissue cultures established as monolayers with tight junctions and differentiated cell composition, with both types of cultures comprising ciliated and non-ciliated epithelial cells. Fibroblasts were also frequently found in explant cultures but rarely seen in dissociated tissue cultures. In both culture systems, the highest ciliated cell density appeared at 7th–10th culture day and declined with time, with the lifespan of ciliated cells ranging from 14 to 21 days. Overall, 10% of the cells in explant cultures and 20% of the cells in the dissociated tissue cultures were ciliated. These two cultures demonstrated similar ciliary beat frequency values at baseline (7.78±1.99 Hz and 7.91±2.52 Hz, respectively) and reacted equivalently following stimulation with 100 μM ATP. The results of this study indicate that both the explant outgrowth and dissociated tissue culture techniques are suitable for growing well-differentiated nasal ciliated and non-ciliated cells, which have growth characteristics and ciliary activity similar to those of nasal epithelial cells in vivo.
关键词: ciliated cells ciliary beat frequency dissociated tissue culture explant culture nasal epithelial cells
Effects of red and blue LEDs on
Lili CHEN, Xuzhang XUE, Yadong YANG, Fei CHEN, Jie ZHAO, Xiquan WANG, Alam Tariful KHAN, Yuegao HU
《农业科学与工程前沿(英文)》 2018年 第5卷 第2期 页码 197-205 doi: 10.15302/J-FASE-2018224
关键词: blue LED microtuber plant tissue culture potato plantlets in vitro red LED
Atypical pituitary hormone–target tissue axis
《医学前沿(英文)》 2023年 第17卷 第1期 页码 1-17 doi: 10.1007/s11684-022-0973-7
关键词: thyroid-stimulating hormone follicle-stimulating hormone luteinizing hormone adrenocorticotrophic hormone prolactin
Progress and perspectives of neural tissue engineering
null
《医学前沿(英文)》 2015年 第9卷 第4期 页码 401-411 doi: 10.1007/s11684-015-0415-x
Traumatic injuries to the nervous system lead to a common clinical problem with a quite high incidence and affect the patient’s quality of life. Based on a major challenge not yet addressed by current therapeutic interventions for these diseases, a novel promising field of neural tissue engineering has emerged, grown, and attracted increasing interest. This review provides a brief summary of the recent progress in the field, especially in combination with the research experience of the author’s group. Several important aspects related to tissue engineered nerves, including the theory on their construction, translation into the clinic, improvements in fabrication technologies, and the formation of a regenerative environment, are delineated and discussed. Furthermore, potential research directions for the future development of neural tissue engineering are suggested.
Key stakeholders’ perspectives on the ideal partnering culture in construction projects
Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC
《工程管理前沿(英文)》 2022年 第9卷 第2期 页码 312-325 doi: 10.1007/s42524-020-0135-z
关键词: project culture organisational culture partnering construction culture stakeholder perspectives German
The stem cell and tissue engineering research in Chinese ophthalmology
GE Jian, LIU Jingbo
《医学前沿(英文)》 2007年 第1卷 第1期 页码 6-10 doi: 10.1007/s11684-007-0002-x
关键词: available observable neuroregeneration protection function reconstruction
Zhou-Fang XIONG MD, Wei-Hong DONG MD, Ze-Hua WANG MD,
《医学前沿(英文)》 2010年 第4卷 第1期 页码 112-116 doi: 10.1007/s11684-010-0016-7
关键词: cervical cancer radical surgery peritoneum subcutaneous tissue
Primary cilia in hard tissue development and diseases
《医学前沿(英文)》 2021年 第15卷 第5期 页码 657-678 doi: 10.1007/s11684-021-0829-6
关键词: primary cilia bone mechanical sensing hard tissue cilium-related bone disease tooth
Kishore Gopalakrishnan, Javad Roostaei, Yongli Zhang
《环境科学与工程前沿(英文)》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1075-2
RSM is used to explore the impact of different parameter on algal growth response. Mixed algal culture promotes algal biomass and lipid accumulation. Optimized conditions achieve maximum productivity of algal biomass and lipid.
关键词: Algal biofuels Algal mixed cultures Algal biomass Algal lipid Wastewater Response surface methodology
Construction engineering management culture shift: Is the lowest tender offer dead?
Eric SCHEEPBOUWER, Douglas D. GRANSBERG, Carla Lopez del PUERTO
《工程管理前沿(英文)》 2017年 第4卷 第1期 页码 49-57 doi: 10.15302/J-FEM-2017014
关键词: cost certainty lowest responsible bid best value public procurement construction engineering management culture
Stem cell niches and endogenous electric fields in tissue repair
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 40-44 doi: 10.1007/s11684-011-0108-z
Adult stem cells are responsible for homeostasis and repair of many tissues. Endogenous adult stem cells reside in certain regions of organs, known as the stem cell niche, which is recognized to have an important role in regulating tissue maintenance and repair. In wound healing and tissue repair, stem cells are mobilized and recruited to the site of wound, and participate in the repair process. Many regulatory factors are involved in the stem cell-based repair process, including stem cell niches and endogenous wound electric fields, which are present at wound tissues and proved to be important in guiding wound healing. Here we briefly review the role of stem cell niches and endogenous electric fields in tissue repair, and hypothesize that endogenous electric fields become part of stem cell niche in the wound site.
Microorganism-derived biological macromolecules for tissue engineering
《医学前沿(英文)》 2022年 第16卷 第3期 页码 358-377 doi: 10.1007/s11684-021-0903-0
关键词: biological macromolecules regenerative medicine tissue engineering exopolysaccharide carbohydrate
Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 415-420 doi: 10.1007/s11684-009-0072-z
关键词: tissue inhibitor of metalloproteinase-3 intervertebral disc rabbit gene therapy
标题 作者 时间 类型 操作
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue
null
期刊论文
Effects of red and blue LEDs on
Lili CHEN, Xuzhang XUE, Yadong YANG, Fei CHEN, Jie ZHAO, Xiquan WANG, Alam Tariful KHAN, Yuegao HU
期刊论文
Key stakeholders’ perspectives on the ideal partnering culture in construction projects
Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC
期刊论文
Non-closure of the peritoneum and subcutaneous tissue at radical hysterectomy: A randomized controlled
Zhou-Fang XIONG MD, Wei-Hong DONG MD, Ze-Hua WANG MD,
期刊论文
Construction engineering management culture shift: Is the lowest tender offer dead?
Eric SCHEEPBOUWER, Douglas D. GRANSBERG, Carla Lopez del PUERTO
期刊论文